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e coli  (BPS Bioscience)


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    Structured Review

    BPS Bioscience e coli
    E Coli, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/his+plpro/pm39818814-281-9-17?v=BPS+Bioscience
    Average 93 stars, based on 10 article reviews
    e coli - by Bioz Stars, 2026-06
    93/100 stars

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    Image Search Results


    Inhibition of SARS-CoV-2 PLpro enzyme activities by 9-AMN. The enzymatic inhibitory effects of 9-AMN and minocycline (each at 50 µM) on the SARS-CoV-2 PLpro enzyme were evaluated as detailed in the materials and methods. ( A ) Inhibition of proteolytic activity. ( B ) Inhibition of DUB activity. ( C ) Gradual reduction of proteolytic activity with increasing 9-AMN concentration. ( D ) Gradual decline of DUB activity with increasing 9-AMN concentration. ( E ) Inhibition of 3CLpro enzyme activity ( F ) Inhibition of RdRp enzyme activity. The enzymatic activity of control samples treated with DMSO (0.1%) is taken as 100% for calculating relative enzymatic activity. GRL-0617 (50 µM) treated wells were used as a specificity control. The graph is representative of three independent experiments (n = 3). The P value < 0.05 is denoted as

    Journal: Scientific Reports

    Article Title: 9-aminominocycline potentiates the efficacy of EIDD-1931 and PF-332 by targeting the papain like protease enzyme of SARS-CoV-2

    doi: 10.1038/s41598-025-89717-3

    Figure Lengend Snippet: Inhibition of SARS-CoV-2 PLpro enzyme activities by 9-AMN. The enzymatic inhibitory effects of 9-AMN and minocycline (each at 50 µM) on the SARS-CoV-2 PLpro enzyme were evaluated as detailed in the materials and methods. ( A ) Inhibition of proteolytic activity. ( B ) Inhibition of DUB activity. ( C ) Gradual reduction of proteolytic activity with increasing 9-AMN concentration. ( D ) Gradual decline of DUB activity with increasing 9-AMN concentration. ( E ) Inhibition of 3CLpro enzyme activity ( F ) Inhibition of RdRp enzyme activity. The enzymatic activity of control samples treated with DMSO (0.1%) is taken as 100% for calculating relative enzymatic activity. GRL-0617 (50 µM) treated wells were used as a specificity control. The graph is representative of three independent experiments (n = 3). The P value < 0.05 is denoted as "*" indicating statistical significance, while “ns” refers to statistically not significant.

    Article Snippet: The recombinant full-length untagged 3CLpro (cat#100823) and His-tagged PLpro (cat#100735), assay buffers (cat#79956 and cat#78039-1 for 3CLpro and PLpro, respectively), GC-367 (cat#78013), and fluorescently labeled substrates (cat#79952 for 3CLpro, cat#79997 for PLpro protease activity, and cat#82200 for DUB activity) were purchased from BPS Biosciences (San Diego, CA, USA).

    Techniques: Inhibition, Activity Assay, Concentration Assay, Control